Tissue engineering aims to fabricate functional tissue for applications in regenerative medicine and drug testing. Soft tissue engineering comprises three primary components used with the aim of restoring, maintaining, or replacing organs. Three-dimensional (3D) printing is a versatile technique used for various tissue-engineering applications and regenerative medicine. 3D printing techniques have significant capability in creating 3D scaffolds for tissue engineering and employing variety of biomaterials for scaffold fabrication. 3D scaffolds have an important role in cellular attachment, proliferation, nutrient transportation and vascularization. This research aims to development 3D printing techniques in soft-tissue engineering (cardiovascular system, nerves, cartilage, liver, skin and trachea) through novel fabrication methods of soft tissue engineering constructs.